We study membrane protein science at single-molecule resolution, hopefully contributing to pertinent areas of pharmacy and medicine. Proteins classified as membrane proteins are the key targets of commercial drugs (~50% of drugs), and thus better understanding of their hidden chemistry and physics cannot be more emphasized to develop effective strategies of therapeutic intervention. However, the progress is relatively slow due to the technical challenges in handling the recalcitrant membrane proteins. The vision of our laboratory is not only to understand their fundamental bio-physico-chemical principles such as misfolding disease mechanisms at single-molecule level, but also to develop new methods tackling the notorious.